Zymoprep Yeast Plasmid Miniprep II His-tagged Protein Purification without using toxic phenol-chloroform or

without using toxic phenol-chloroform or cumbersome protein precipitation and removal steps

Binding conditions are adjusted using this buffer to selectively recover ≥ 300 bp

This simple procedure is based on the use of a unique single-buffer system and Zymo-Spin column technology that allows for selective recovery of total RNA (> 17 nt)

Coli transformation

ZymoBIOMICS MagWash 1 is designed to be used with the ZymoBIOMICS DNA MagBead kits (D4308)

Zymoprep Yeast Plasmid Miniprep II His-tagged Protein Purification without using toxic phenol-chloroform orThe Zymoprep Yeast Plasmid Miniprep II is a simple and efficient yeast plasmid miniprep that is based on the E. coli alkaline lysis method but using Zymolyase as the first solution. The kit provides all the necessary reagents for plasmid isolation from S. cerevisiae, C. albicans and S. pombe, and any fungi whose cell walls are susceptible to yeast lytic enzyme lysis. The procedure is simple and efficient, with no need for glass beads or phenol. The